#6258
Store at -20°C
ETS-1 Antibody
New 06/12
n 100 µl
(10 western blots)
W, IP
Endogenous
H, M, R 52 kDa Rabbit**
Background: ETS-1 is a proto-oncoprotein that belongs
to the E26 Transformation-specific Sequence (ETS) family
of transcription factors that share a unique and highly
conserved DNA binding domain (1). ETS-1 plays important
roles in vascular development and angiogenesis (2), and
vascular inflammation and remodeling (3). The target genes
of ETS-1 include receptor tyrosine kinases, MMPs, and cell
adhesion molecules (4-6). In addition, ETS-1 is involved in
regulation of energy metabolism in cancer cells (7). ETS-1
activity is regulated by two different types of phosphoryla-
tion sites. While phosphorylation at a cluster of serine
residues in the exon VII domain by CaMKII inhibits ETS-1
DNA binding activity (8), phosphorylation at its Thr38 site
by Ras activates ETS-1 (9).
Specificity/Sensitivity: ETS-1 Antibody recognizes
endogenous levels of total ETS-1 protein.
Source/Purification: Polyclonal antibodies are produced
by immunizing animals with a synthetic peptide corre-
sponding to residues surrounding Ser179 of human ETS-1
protein. Antibodies are purified by protein A and peptide
affinity chromatography.
Background References:
(1) Bartel, F.O. et al. (2000) Oncogene 19, 6443-54.
(2) Sato, Y. (2001) Cell Struct Funct 26, 19-24.
(3) Oettgen, P. (2006) Circ Res 99, 1159-66.
(4) Dube, A. et al. (1999) Circ Res 84, 1177-85.
(5) Lelièvre, E. et al. (2000) Oncogene 19, 2438-46.
(6) Ghosh, S. et al. (2012) J Biol Chem 287, 15001-15.
(7) Verschoor, M.L. et al. (2010) PLoS One 5, e13565.
(8) Pognonec, P. et al. (1988) EMBO J 7, 977-83.
(9) Yang, B.S. et al. (1996) Mol Cell Biol 16, 538-47.
Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM
NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C.
Do not aliquot the antibody.
*Species cross-reactivity is determined by western blot.
** Anti-rabbit secondary antibodies must be used to
detect this antibody.
Recommended Antibody Dilutions:
Western blotting 1:1000
Immunoprecipitation 1:50
For product specific protocols please see the web page
for this product at www.cellsignal.com.
Please visit www.cellsignal.com for a complete listing
of recommended complementary products.
Orders
n
877-616-CELL (2355)
Support
n
877-678-TECH (8324)
Web
n
www.cellsignal.com
Applications Species Cross-Reactivity* Molecular Wt. Source
Entrez-Gene ID #2113
Swiss-Prot Acc. #P14921
Species Cross-Reactivity Key: H—human M—mouse R—rat Hm—hamster Mk—monkey Mi—mink C—chicken Dm—D. melanogaster X—Xenopus Z—zebrafish B—bovine
Dg—dog Pg—pig Sc—S. cerevisiae Ce—C. elegans Hr—horse All—all species expected
Species enclosed in parentheses are predicted to react based on 100% homology.
Applications Key: W—Western IP—Immunoprecipitation IHC—Immunohistochemistry ChIP—Chromatin Immunoprecipitation IF—Immunofluorescence F—Flow cytometry E-P—ELISA-Peptide
IMPORTANT: For western blots, incubate membrane with diluted antibody in 5% w/v BSA, 1X TBS,
0.1% Tween-20 at 4°C with gentle shaking, overnight.
For Research Use Only. Not For Use In Diagnostic Procedures.
© 2012 Cell Signaling Technology, Inc.
Cell Signaling Technology
®
is a trademark of Cell Signaling Technology, Inc.
3
kDa
ETS-1
RL-7
A20
OVCAR8
C6
200
140
100
80
60
50
40
30
10
20
Western blot analysis of extracts from various cell lines using
ETS-1 Antibody.
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